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Siva Subramanian, N.
- Evaluation of Acute and Subacute Toxicity Studies of Polyherbal Extract on Rodents
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Materials and Methods: Herbs were mixed equal in proportion and extracted with ethanol. The acute and subacute toxicity studies on ethanolic extract of were carried out to evaluate the safety on rodents. Study on acute toxicity of the ethanolic extract was found to be safe at the dose of 2000 mg/kg body weight orally as per OECD guideline No.423. General behaviour adverse effects and mortality were determined for upto 14 days. In the subacute study, the extract was administered orally at doses of 100, 200 and 400 mg/kg once in a week for 6 weeks to rats. Biochemical, haematological and histological parameters were determined after 6 weeks.
Results: In the acute study there was no toxicity/death was observed at the dose of 2000 mg/kg body weight. The onset of toxicity and signs of toxicity was also not there. In subacute study, no significant treatment related changes in the levels of haematological, hepatic and renal parameters were observed at the end of the study. It suggests that the polyherbal ethanolic extract does not appear to have any significant toxicity. Hence, the extract was safe without any toxic symptoms and signs can be further used for the pharmacological screening of antidepressant action based upon the traditional knowledge and usage.
Authors
Affiliations
1 Department of Pharmacology, Teegala Krishna Reddy College of Pharmacy, Medbowli, Meerpet, Saroor nagar (M), Hyderabad-97, IN
2 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641404, IN
1 Department of Pharmacology, Teegala Krishna Reddy College of Pharmacy, Medbowli, Meerpet, Saroor nagar (M), Hyderabad-97, IN
2 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore-641404, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 6, No 2 (2014), Pagination: 94-100Abstract
Background: Cycas circinalis (flowers), Artemisia absinthium (whole plant) and Nardostachys jatamansi (rhizomes) were used traditionally for various ailments, curing diseases and majorly in CNS disorders in the treatment of antidepressant. The objective of this study was to investigate the toxicity of the ethanolic extract of selected herbs on rodents and to screen its antidepressant action in further research.Materials and Methods: Herbs were mixed equal in proportion and extracted with ethanol. The acute and subacute toxicity studies on ethanolic extract of were carried out to evaluate the safety on rodents. Study on acute toxicity of the ethanolic extract was found to be safe at the dose of 2000 mg/kg body weight orally as per OECD guideline No.423. General behaviour adverse effects and mortality were determined for upto 14 days. In the subacute study, the extract was administered orally at doses of 100, 200 and 400 mg/kg once in a week for 6 weeks to rats. Biochemical, haematological and histological parameters were determined after 6 weeks.
Results: In the acute study there was no toxicity/death was observed at the dose of 2000 mg/kg body weight. The onset of toxicity and signs of toxicity was also not there. In subacute study, no significant treatment related changes in the levels of haematological, hepatic and renal parameters were observed at the end of the study. It suggests that the polyherbal ethanolic extract does not appear to have any significant toxicity. Hence, the extract was safe without any toxic symptoms and signs can be further used for the pharmacological screening of antidepressant action based upon the traditional knowledge and usage.
Keywords
Cycas circinalis, Artemisia absinthium, Nardostachys jatamansi, Acute and Subacute Toxicity, Biochemical, Haematological, Histological, Ethanolic Extract.
References
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- Anxiolytic Effect of Polyherbal Extracts on Rodents
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Authors
Affiliations
1 Department of Pharmacology, Teegala Krishna Reddy College of Pharmacy, Meerpet, Hyderabad, Andhra Pradesh, IN
2 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamil Nadu, IN
1 Department of Pharmacology, Teegala Krishna Reddy College of Pharmacy, Meerpet, Hyderabad, Andhra Pradesh, IN
2 RVS College of Pharmaceutical Sciences, Sulur, Coimbatore, Tamil Nadu, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 7, No 1 (2015), Pagination: 1-5Abstract
Background: Anxiety, unlike other psychiatric conditions such as schizophrenia or depression, is both an emotional and a psychiatric disorder. It commonly co-exists with other clinical conditions but can be diagnosed on the basis of symptoms and course of the disease.
Objective: To study the anxiolytic activity of polyherbal extract and to estimate the biogenic amines.
Materials and Methods: The anxiolytic effect of polyherbal extracts containing Leptadenia reticulata ischolar_mains (Asclepiadaceae), Mimusops elengi bark (Sapotaceae) and Evolvulus alsinoides Whole plant (Convolvulaceae) were studied in rodents. Different animal models like elevated plus maze, stair case, and mirrored chamber were used for assessing the anxiolytic property of the polyherbal extracts.
Results: The results revealed that ethanolic and acetone extract at the dose of 200mg/kg significantly produced anxiolytic action, in a same way to that of diazepam (2mg/kg, p.o) standard drug, along with significant entries responses in all the above experimental models used. Polyherbal extracts showed significant (p<0.001) decreases in serotonin and norepinephrine in the whole brain of rat. The dopamine level was significantly (p<0.001) increased by the extracts. The extracts might potentially act by increased GABAnergic activation and/or by modulating the serotoninergic levels in the central nervous system.
Conclusion: Traditional system of medicine using herbs in neuro disorders exhibit potent anxiolytic action in the above study.